mouse chk1 Search Results


pchk1 s317  (R&D Systems)
93
R&D Systems pchk1 s317
Pchk1 S317, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pchk1 s317/product/R&D Systems
Average 93 stars, based on 1 article reviews
pchk1 s317 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
human/mouse/rat chk1 antibody  (Bio-Techne corporation)
94
Bio-Techne corporation human/mouse/rat chk1 antibody
Human/Mouse/Rat Chk1 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human/mouse/rat chk1 antibody/product/Bio-Techne corporation
Average 94 stars, based on 1 article reviews
human/mouse/rat chk1 antibody - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
antibodies against total chk1  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc antibodies against total chk1
Antibodies Against Total Chk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against total chk1/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
antibodies against total chk1 - by Bioz Stars, 2026-03
97/100 stars
  Buy from Supplier
goat anti chk1  (R&D Systems)
90
R&D Systems goat anti chk1
Goat Anti Chk1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti chk1/product/R&D Systems
Average 90 stars, based on 1 article reviews
goat anti chk1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mouse anti-chk1  (Biomeda corporation)
90
Biomeda corporation mouse anti-chk1
ATM and ATR are involved in p53 phosphorylation at serine 15 by CDK2 inhibition. (A) A2780 cells were treated with the indicated inhibitors for 4 h. Compound 25 was subsequently added to the medium for an additional 4 h. PI3K inhibitors caffeine and LY294002 effectively blocked compound 25-induced p53 phosphorylation and accumulation, while p38 inhibitors SB202190 (SB1), SB203580 (SB2), and MEK inhibitors (PD98059 and U0126) had no effect. (B and C) After either treatment with compound 25 or transfection of CDK2 siRNA, cell lysates were incubated with a specific phospho-serine/threonine ATM/ATR substrate antibody (Ab) and subject to immunoprecipitation (IP). p53, <t>CHK1,</t> and NBS1 were phosphorylated and immunoprecipitated by this antibody. Luciferase (Luc) siRNA transfection was used as a control for CDK2 siRNA transfection. (D) H2AX phosphorylation at serine 139 (γ-H2AX) was induced by compound 25, roscovitine, and olomoucine. (E) A2780 cells were treated with DMSO and compound 25 (CPD25) for 4 h. Samples were blotted with phospho-CHK1 (serine 345) or phospho-CHK2 (threonine 68) antibody. Total levels of CHK1 and CHK2 were also shown. (F) A2780 cells were treated with DMSO or compound 25 for 4 h. Samples were blotted with phospho-ATM (serine 1981) or ATM antibody.
Mouse Anti Chk1, supplied by Biomeda corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-chk1/product/Biomeda corporation
Average 90 stars, based on 1 article reviews
mouse anti-chk1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
anti-chk1 (am7401a)  (WuXi AppTec)
90
WuXi AppTec anti-chk1 (am7401a)
(A) Cell cycle analysis. CD36 + EPCs were treated with VE821 at 3 h prior to NS1-expressing lentivirus transduction or mock transduction. After 48 h, cells were collected, and the cell cycle phase of NS1-expressing cells (selected by staining with anti-Flag) was examined by flow cytometry. (B) Statistical analyses. The percentage of cells treated with DMSO or VE821 that were at G1-, S-, and G2-phase is depicted in color. The numbers shown are the percentages of the cells at G2-phase, and are statistically compared as indicated. ** P<0.01. (C-E) Western blot analysis. (C) CD36 + EPCs were either treated with DMSO or VE821 at 3 h prior to lentivirus or mock transduction. After 48 h, cells were collected for Western blotting to detect ATR(pT1989). (D&E) CD36 + EPCs were transduced with Lenti-NS1 or Lenti-NS1 mTAD2 . After 48 h, cells were collected for Western blotting to detect ATR(pT1989), <t>CHK1(pS345),</t> CDC25C(pS216), CDK1(pY15), and β-actin. Cells treated with HU or Noco at 24 h prior to analysis were used as controls.
Anti Chk1 (Am7401a), supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-chk1 (am7401a)/product/WuXi AppTec
Average 90 stars, based on 1 article reviews
anti-chk1 (am7401a) - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mouse monoclonal chk1  (Gentex Corporation)
90
Gentex Corporation mouse monoclonal chk1
(A) Cell cycle analysis. CD36 + EPCs were treated with VE821 at 3 h prior to NS1-expressing lentivirus transduction or mock transduction. After 48 h, cells were collected, and the cell cycle phase of NS1-expressing cells (selected by staining with anti-Flag) was examined by flow cytometry. (B) Statistical analyses. The percentage of cells treated with DMSO or VE821 that were at G1-, S-, and G2-phase is depicted in color. The numbers shown are the percentages of the cells at G2-phase, and are statistically compared as indicated. ** P<0.01. (C-E) Western blot analysis. (C) CD36 + EPCs were either treated with DMSO or VE821 at 3 h prior to lentivirus or mock transduction. After 48 h, cells were collected for Western blotting to detect ATR(pT1989). (D&E) CD36 + EPCs were transduced with Lenti-NS1 or Lenti-NS1 mTAD2 . After 48 h, cells were collected for Western blotting to detect ATR(pT1989), <t>CHK1(pS345),</t> CDC25C(pS216), CDK1(pY15), and β-actin. Cells treated with HU or Noco at 24 h prior to analysis were used as controls.
Mouse Monoclonal Chk1, supplied by Gentex Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal chk1/product/Gentex Corporation
Average 90 stars, based on 1 article reviews
mouse monoclonal chk1 - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mus musculus chk1 coding sequence cds  (Sino Biological)
94
Sino Biological mus musculus chk1 coding sequence cds
(A) Cell cycle analysis. CD36 + EPCs were treated with VE821 at 3 h prior to NS1-expressing lentivirus transduction or mock transduction. After 48 h, cells were collected, and the cell cycle phase of NS1-expressing cells (selected by staining with anti-Flag) was examined by flow cytometry. (B) Statistical analyses. The percentage of cells treated with DMSO or VE821 that were at G1-, S-, and G2-phase is depicted in color. The numbers shown are the percentages of the cells at G2-phase, and are statistically compared as indicated. ** P<0.01. (C-E) Western blot analysis. (C) CD36 + EPCs were either treated with DMSO or VE821 at 3 h prior to lentivirus or mock transduction. After 48 h, cells were collected for Western blotting to detect ATR(pT1989). (D&E) CD36 + EPCs were transduced with Lenti-NS1 or Lenti-NS1 mTAD2 . After 48 h, cells were collected for Western blotting to detect ATR(pT1989), <t>CHK1(pS345),</t> CDC25C(pS216), CDK1(pY15), and β-actin. Cells treated with HU or Noco at 24 h prior to analysis were used as controls.
Mus Musculus Chk1 Coding Sequence Cds, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mus musculus chk1 coding sequence cds/product/Sino Biological
Average 94 stars, based on 1 article reviews
mus musculus chk1 coding sequence cds - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
adeno-associated virus 9 vectors expressing mouse chk1 shrna  (Vigene Biosciences)
90
Vigene Biosciences adeno-associated virus 9 vectors expressing mouse chk1 shrna
(A) Cell cycle analysis. CD36 + EPCs were treated with VE821 at 3 h prior to NS1-expressing lentivirus transduction or mock transduction. After 48 h, cells were collected, and the cell cycle phase of NS1-expressing cells (selected by staining with anti-Flag) was examined by flow cytometry. (B) Statistical analyses. The percentage of cells treated with DMSO or VE821 that were at G1-, S-, and G2-phase is depicted in color. The numbers shown are the percentages of the cells at G2-phase, and are statistically compared as indicated. ** P<0.01. (C-E) Western blot analysis. (C) CD36 + EPCs were either treated with DMSO or VE821 at 3 h prior to lentivirus or mock transduction. After 48 h, cells were collected for Western blotting to detect ATR(pT1989). (D&E) CD36 + EPCs were transduced with Lenti-NS1 or Lenti-NS1 mTAD2 . After 48 h, cells were collected for Western blotting to detect ATR(pT1989), <t>CHK1(pS345),</t> CDC25C(pS216), CDK1(pY15), and β-actin. Cells treated with HU or Noco at 24 h prior to analysis were used as controls.
Adeno Associated Virus 9 Vectors Expressing Mouse Chk1 Shrna, supplied by Vigene Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adeno-associated virus 9 vectors expressing mouse chk1 shrna/product/Vigene Biosciences
Average 90 stars, based on 1 article reviews
adeno-associated virus 9 vectors expressing mouse chk1 shrna - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
mouse chk1 gene orf cdna clone in cloning vector  (Sino Biological)
94
Sino Biological mouse chk1 gene orf cdna clone in cloning vector
(A) Cell cycle analysis. CD36 + EPCs were treated with VE821 at 3 h prior to NS1-expressing lentivirus transduction or mock transduction. After 48 h, cells were collected, and the cell cycle phase of NS1-expressing cells (selected by staining with anti-Flag) was examined by flow cytometry. (B) Statistical analyses. The percentage of cells treated with DMSO or VE821 that were at G1-, S-, and G2-phase is depicted in color. The numbers shown are the percentages of the cells at G2-phase, and are statistically compared as indicated. ** P<0.01. (C-E) Western blot analysis. (C) CD36 + EPCs were either treated with DMSO or VE821 at 3 h prior to lentivirus or mock transduction. After 48 h, cells were collected for Western blotting to detect ATR(pT1989). (D&E) CD36 + EPCs were transduced with Lenti-NS1 or Lenti-NS1 mTAD2 . After 48 h, cells were collected for Western blotting to detect ATR(pT1989), <t>CHK1(pS345),</t> CDC25C(pS216), CDK1(pY15), and β-actin. Cells treated with HU or Noco at 24 h prior to analysis were used as controls.
Mouse Chk1 Gene Orf Cdna Clone In Cloning Vector, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse chk1 gene orf cdna clone in cloning vector/product/Sino Biological
Average 94 stars, based on 1 article reviews
mouse chk1 gene orf cdna clone in cloning vector - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier

Image Search Results


ATM and ATR are involved in p53 phosphorylation at serine 15 by CDK2 inhibition. (A) A2780 cells were treated with the indicated inhibitors for 4 h. Compound 25 was subsequently added to the medium for an additional 4 h. PI3K inhibitors caffeine and LY294002 effectively blocked compound 25-induced p53 phosphorylation and accumulation, while p38 inhibitors SB202190 (SB1), SB203580 (SB2), and MEK inhibitors (PD98059 and U0126) had no effect. (B and C) After either treatment with compound 25 or transfection of CDK2 siRNA, cell lysates were incubated with a specific phospho-serine/threonine ATM/ATR substrate antibody (Ab) and subject to immunoprecipitation (IP). p53, CHK1, and NBS1 were phosphorylated and immunoprecipitated by this antibody. Luciferase (Luc) siRNA transfection was used as a control for CDK2 siRNA transfection. (D) H2AX phosphorylation at serine 139 (γ-H2AX) was induced by compound 25, roscovitine, and olomoucine. (E) A2780 cells were treated with DMSO and compound 25 (CPD25) for 4 h. Samples were blotted with phospho-CHK1 (serine 345) or phospho-CHK2 (threonine 68) antibody. Total levels of CHK1 and CHK2 were also shown. (F) A2780 cells were treated with DMSO or compound 25 for 4 h. Samples were blotted with phospho-ATM (serine 1981) or ATM antibody.

Journal:

Article Title: Intra-S-Phase Checkpoint Activation by Direct CDK2 Inhibition

doi: 10.1128/MCB.24.14.6268-6277.2004

Figure Lengend Snippet: ATM and ATR are involved in p53 phosphorylation at serine 15 by CDK2 inhibition. (A) A2780 cells were treated with the indicated inhibitors for 4 h. Compound 25 was subsequently added to the medium for an additional 4 h. PI3K inhibitors caffeine and LY294002 effectively blocked compound 25-induced p53 phosphorylation and accumulation, while p38 inhibitors SB202190 (SB1), SB203580 (SB2), and MEK inhibitors (PD98059 and U0126) had no effect. (B and C) After either treatment with compound 25 or transfection of CDK2 siRNA, cell lysates were incubated with a specific phospho-serine/threonine ATM/ATR substrate antibody (Ab) and subject to immunoprecipitation (IP). p53, CHK1, and NBS1 were phosphorylated and immunoprecipitated by this antibody. Luciferase (Luc) siRNA transfection was used as a control for CDK2 siRNA transfection. (D) H2AX phosphorylation at serine 139 (γ-H2AX) was induced by compound 25, roscovitine, and olomoucine. (E) A2780 cells were treated with DMSO and compound 25 (CPD25) for 4 h. Samples were blotted with phospho-CHK1 (serine 345) or phospho-CHK2 (threonine 68) antibody. Total levels of CHK1 and CHK2 were also shown. (F) A2780 cells were treated with DMSO or compound 25 for 4 h. Samples were blotted with phospho-ATM (serine 1981) or ATM antibody.

Article Snippet: Rabbit anti-CDK2, rabbit anti-cyclin E (Upstate Biotechnology, Lake Placid, N.Y.), rabbit anti-cyclin A, rabbit anti-p21, mouse anti-CHK2, goat anti-ATR, goat anti-replication protein A (RPA) p70 (Santa Cruz Biotechnology, Santa, Cruz, Calif.), mouse anti-CHK1 (Biomeda, Foster City, Calif.), mouse anti-p53, rabbit anti-ATR (Oncogene, San Diego, Calif.), rabbit anti-ATM (Oncogene and Santa Cruz Biotechnology), rabbit anti-ORC2, rabbit anti-minichromosome maintenance protein 2 (anti-MCM2), rabbit anti-MCM3, rabbit anti-MCM4, rabbit anti-MCM5, rabbit anti-MCM6, and rabbit anti-MCM7 (BD Biosciences, San Jose, Calif.) antibodies were used for immunoprecipitation or Western blot analysis.

Techniques: Inhibition, Transfection, Incubation, Immunoprecipitation, Luciferase

(A) Cell cycle analysis. CD36 + EPCs were treated with VE821 at 3 h prior to NS1-expressing lentivirus transduction or mock transduction. After 48 h, cells were collected, and the cell cycle phase of NS1-expressing cells (selected by staining with anti-Flag) was examined by flow cytometry. (B) Statistical analyses. The percentage of cells treated with DMSO or VE821 that were at G1-, S-, and G2-phase is depicted in color. The numbers shown are the percentages of the cells at G2-phase, and are statistically compared as indicated. ** P<0.01. (C-E) Western blot analysis. (C) CD36 + EPCs were either treated with DMSO or VE821 at 3 h prior to lentivirus or mock transduction. After 48 h, cells were collected for Western blotting to detect ATR(pT1989). (D&E) CD36 + EPCs were transduced with Lenti-NS1 or Lenti-NS1 mTAD2 . After 48 h, cells were collected for Western blotting to detect ATR(pT1989), CHK1(pS345), CDC25C(pS216), CDK1(pY15), and β-actin. Cells treated with HU or Noco at 24 h prior to analysis were used as controls.

Journal: PLoS Pathogens

Article Title: Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway

doi: 10.1371/journal.ppat.1006266

Figure Lengend Snippet: (A) Cell cycle analysis. CD36 + EPCs were treated with VE821 at 3 h prior to NS1-expressing lentivirus transduction or mock transduction. After 48 h, cells were collected, and the cell cycle phase of NS1-expressing cells (selected by staining with anti-Flag) was examined by flow cytometry. (B) Statistical analyses. The percentage of cells treated with DMSO or VE821 that were at G1-, S-, and G2-phase is depicted in color. The numbers shown are the percentages of the cells at G2-phase, and are statistically compared as indicated. ** P<0.01. (C-E) Western blot analysis. (C) CD36 + EPCs were either treated with DMSO or VE821 at 3 h prior to lentivirus or mock transduction. After 48 h, cells were collected for Western blotting to detect ATR(pT1989). (D&E) CD36 + EPCs were transduced with Lenti-NS1 or Lenti-NS1 mTAD2 . After 48 h, cells were collected for Western blotting to detect ATR(pT1989), CHK1(pS345), CDC25C(pS216), CDK1(pY15), and β-actin. Cells treated with HU or Noco at 24 h prior to analysis were used as controls.

Article Snippet: The following first antibodies were purchased: anti-Strep (A01732) from GenScript, Piscataway, NJ; mouse anti-BrdU (clone B44) (347580), anti-cyclin B1 (554179), anti-CDK1 (pY15) (612306) from BD, San Jose, CA; rabbit anti-BrdU (600-401-C29) and mouse anti-Flag (2001-301-1313) from Rockland, Limerick, PA; anti-CDK1 (A303-663A), anti-CDC25C (A301-390A), anti-MYT1 (A302-424A), anti-p38 (A300-707A) from Bethyl, Montgomery, TX; anti-CDK1 (pT161) (P12-1090) from Assay Biotech, Sunnyvale, CA; anti-CDC25C(pS216) (4204), anti-γH2AX(pS139) (05–636), anti-B19V capsid (MAB8292) from Millipore, Billerica, MA; anti-WEE1 (AP8106b), anti-CHK1 (AM7401a), anti-CHK2 (AP4999a), anti-MAPKAPK2(pS272) (AP3147a) from Abgent, San Diego, CA; anti-ATR (ab2905), anti-GAPDH (Ab8245) from Abcam, Cambridge, MA; anti-ATR (pT1989) (GTX128145), anti-CHK1(pS345) (GTX100065S), anti-RPA32(pT21) (GTX62664) from GeneTex, Irvine, CA; anti-ATM (2873), anti-MAPKAPK2(MK2) (12155s), anti-MARK3 (9311s), anti-p21 (2947p), anti-Histone H3 (9715S) from Cell Signaling Technology, Danvers, MA; anti-ATM(pS1981) (2952–1), anti-CHK2(pS33/35) (2297–1) from Epitomics, Burlingame, CA; anti-p38 (pT180/Y182) (p190-1802) from Phosphosolutions, Aurora, CO; anti-β-actin (A5441) from Sigma-Aldrich, St. Louis, MO.

Techniques: Cell Cycle Assay, Expressing, Transduction, Staining, Flow Cytometry, Western Blot

NS1 activates ATR through its TAD domain. Activated ATR then transduces signals to CDC25C through activating CHK1. CDC25C phosphatase activity is negatively regulated by phosphorylation at serine 216, which then creates a binding site for the 14-3-3 protein in the cytoplasm . Thus, inactive CDC25C cannot dephosphorylate the cyclin B1/CDK1(pT14/Y15) complex; the latter component is inactive, and so progression from G2- to M-phase is blocked. It is proposed that DNA replication-induced DDR, and thereafter ATR/CHK1 activation, which plays a role in viral DNA replication, should not be involved in CDC25C phosphorylation (see the section for further explanation). The question mark indicates potential modification of CHK1, in addition to the phosphorylation at S345.

Journal: PLoS Pathogens

Article Title: Parvovirus B19 NS1 protein induces cell cycle arrest at G2-phase by activating the ATR-CDC25C-CDK1 pathway

doi: 10.1371/journal.ppat.1006266

Figure Lengend Snippet: NS1 activates ATR through its TAD domain. Activated ATR then transduces signals to CDC25C through activating CHK1. CDC25C phosphatase activity is negatively regulated by phosphorylation at serine 216, which then creates a binding site for the 14-3-3 protein in the cytoplasm . Thus, inactive CDC25C cannot dephosphorylate the cyclin B1/CDK1(pT14/Y15) complex; the latter component is inactive, and so progression from G2- to M-phase is blocked. It is proposed that DNA replication-induced DDR, and thereafter ATR/CHK1 activation, which plays a role in viral DNA replication, should not be involved in CDC25C phosphorylation (see the section for further explanation). The question mark indicates potential modification of CHK1, in addition to the phosphorylation at S345.

Article Snippet: The following first antibodies were purchased: anti-Strep (A01732) from GenScript, Piscataway, NJ; mouse anti-BrdU (clone B44) (347580), anti-cyclin B1 (554179), anti-CDK1 (pY15) (612306) from BD, San Jose, CA; rabbit anti-BrdU (600-401-C29) and mouse anti-Flag (2001-301-1313) from Rockland, Limerick, PA; anti-CDK1 (A303-663A), anti-CDC25C (A301-390A), anti-MYT1 (A302-424A), anti-p38 (A300-707A) from Bethyl, Montgomery, TX; anti-CDK1 (pT161) (P12-1090) from Assay Biotech, Sunnyvale, CA; anti-CDC25C(pS216) (4204), anti-γH2AX(pS139) (05–636), anti-B19V capsid (MAB8292) from Millipore, Billerica, MA; anti-WEE1 (AP8106b), anti-CHK1 (AM7401a), anti-CHK2 (AP4999a), anti-MAPKAPK2(pS272) (AP3147a) from Abgent, San Diego, CA; anti-ATR (ab2905), anti-GAPDH (Ab8245) from Abcam, Cambridge, MA; anti-ATR (pT1989) (GTX128145), anti-CHK1(pS345) (GTX100065S), anti-RPA32(pT21) (GTX62664) from GeneTex, Irvine, CA; anti-ATM (2873), anti-MAPKAPK2(MK2) (12155s), anti-MARK3 (9311s), anti-p21 (2947p), anti-Histone H3 (9715S) from Cell Signaling Technology, Danvers, MA; anti-ATM(pS1981) (2952–1), anti-CHK2(pS33/35) (2297–1) from Epitomics, Burlingame, CA; anti-p38 (pT180/Y182) (p190-1802) from Phosphosolutions, Aurora, CO; anti-β-actin (A5441) from Sigma-Aldrich, St. Louis, MO.

Techniques: Activity Assay, Binding Assay, Activation Assay, Modification